Use of the polymerase chain reaction for the detection of Chlamydia trachomatis in clinical specimens and its comparison
نویسندگان
چکیده
A polymerase chain reaction (PCR) was developed to detect Chlamydia trachomatis in genital tract specimens. Two sets of primers for the PCR were used; one set amplifies a region of the plasmid present in all C trachomatis strains and the other amplifies a conserved region of the genome coding for the major outer membrane protein. The sensitivity of these PCRs were compared with each other, and with the sensitivities of antigen ELISA, Clearview and culture. Southern blotting and probing was used to increase sensitivity of detection. (Genitourin Med 1995;71:169-171)
منابع مشابه
COMPARISON OF PCR BASED ASSAY AND CELL CULTURE IN DETECTION OF UROGENITA L CHLAMYDIA TRACHOMATIS INFECTION AND DETERMINATION OF THE INHIBITORY RATE O F SPECIMENS UNDER STUDY
An amplification polymerase chain reaction (PCR) test for the direct detection of Chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. During 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specime...
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